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To the Editor:Switzer et al investigated the effect of minocycline on plasma metalloproteinase-9 (MMP9) in acute ischemic stroke in the Minocycline to Improve Neurological Outcome in Stroke (MINOS) trial; they observed lower plasma MMP9 activity using zymography assay among tissue plasminogen activator-treated subjects.1Given the ready availability of enzyme immunoassays for quantitative measurements of MMP9, I am interested why the authors chose zymographic analysis of MMP9 activity for the study cohort instead. Throughout the manuscript, the authors …

Letter by Lee Regarding Article, “Matrix Metalloproteinase-9 in an Exploratory Trial of Intravenous Minocycline for Acute Stroke”