Purification of Cardiomyocytes From Differentiating Pluripotent Stem Cells Using Molecular Beacons That Target Cardiomyocyte-Specific mRNA | Zendy

Kiwon Ban | Zendy; B.M. Wile | Zendy; Sangsung Kim | Zendy; HunJun Park | Zendy; Jaemin Byun | Zendy; Kyu-Won Cho | Zendy; Talib Saafir | Zendy; Ming-Ke Song | Zendy; Shan Ping Yu | Zendy; Mary B. Wagner | Zendy; Gang Bao | Zendy; Youngsup Yoon | Zendy

Open Access

Purification of Cardiomyocytes From Differentiating Pluripotent Stem Cells Using Molecular Beacons That Target Cardiomyocyte-Specific mRNA

Author(s) -

Kiwon Ban,

B.M. Wile,

Sangsung Kim,

HunJun Park,

Jaemin Byun,

Kyu-Won Cho,

Talib Saafir,

Ming-Ke Song,

Shan Ping Yu,

Mary B. Wagner,

Gang Bao,

Youngsup Yoon

Publication year - 2013

Publication title -

circulation

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 7.795

H-Index - 607

eISSN - 1524-4539

pISSN - 0009-7322

DOI - 10.1161/circulationaha.113.004228

Subject(s) - induced pluripotent stem cell , flow cytometry , stem cell , microbiology and biotechnology , myosin , myocyte , medicine , biology , embryonic stem cell , biochemistry , gene

Although methods for generating cardiomyocytes from pluripotent stem cells have been reported, current methods produce heterogeneous mixtures of cardiomyocytes and noncardiomyocyte cells. Here, we report an entirely novel system in which pluripotent stem cell-derived cardiomyocytes are purified by cardiomyocyte-specific molecular beacons (MBs). MBs are nanoscale probes that emit a fluorescence signal when hybridized to target mRNAs.

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