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Scanning electron microscopy is a useful modality to visualize the 3-dimensional structures of tissues at high resolution. In addition, processing the tissue samples can provide further architectural information: The removal of connective tissue around the cardiomyocytes and, conversely, the removal of nonfibrous tissue enable us to clearly observe the cardiomyocytes and connective tissue, respectively. Furthermore, backscattered electron emission after heavy-metal staining can provide higher-quality images of the intracellular architecture.1 We have previously reported the 3-dimensional structure of the human left ventricular myocardium.2 Here, we performed an electron …

Three-Dimensional Architecture of Cardiomyocytes and Connective Tissues in Hypertrophic Cardiomyopathy