Open AccessTracking Monocyte Recruitment and Macrophage Accumulation in Atherosclerotic Plaque Progression Using a Novel hCD68GFP/ApoE −/− Reporter Mouse—Brief Report
Author(s) -
Eileen McNeill,
Asif Iqbal,
D. Jones,
Jyoti Patel,
Patricia Coutinho,
Lewis Taylor,
David R. Greaves,
Keith M. Chan
Publication year - 2016
Publication title -
arteriosclerosis thrombosis and vascular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.007
H-Index - 270
eISSN - 1524-4636
pISSN - 1079-5642
DOI - 10.1161/atvbaha.116.308367
Subject(s) - cd11c , green fluorescent protein , integrin alpha m , inflammation , monocyte , adventitia , ccr2 , adoptive cell transfer , macrophage , flow cytometry , immunology , biology , chemistry , microbiology and biotechnology , pathology , medicine , chemokine , chemokine receptor , immune system , t cell , phenotype , biochemistry , gene , in vitro
To create a model of atherosclerosis using green fluorescent protein (GFP)-targeted monocytes/macrophages, allowing analysis of both endogenous GFP + and adoptively transferred GFP + myeloid cells in arterial inflammation. APPROACH AND RESULTS: hCD68GFP reporter mice were crossed with ApoE -/- mice. Expression of GFP was localized to macrophages in atherosclerotic plaques and in angiotensin II-induced aortic aneurysms and correlated with galectin 3 and mCD68 expression. Flow cytometry confirmed GFP + expression in CD11b + /CD64 + , CD11c + /MHC-II HI , and CD11b + /F4/80 + myeloid cells. Adoptive transfer of GFP + monocytes demonstrated monocyte recruitment to both adventitia and atherosclerotic plaque, throughout the aortic root, within 72 hours. We demonstrated the biological utility of hCD68GFP monocytes by comparing the recruitment of wild-type and CCR2 -/- monocytes to sites of inflammation.
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