Open AccessSodium-Dependent Phosphate Cotransporters and Phosphate-Induced Calcification of Vascular Smooth Muscle Cells
Author(s) -
Matthew H. Crouthamel,
Wei Ling Lau,
Elizabeth M. Leaf,
Nicholas W. Chavkin,
Mary C. Wallingford,
Danielle F. Peterson,
Xianwu Li,
Yonggang Liu,
Michael T. Chin,
Moshe Levi,
Cecilia M. Giachelli
Publication year - 2013
Publication title -
arteriosclerosis thrombosis and vascular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.007
H-Index - 270
eISSN - 1524-4636
pISSN - 1079-5642
DOI - 10.1161/atvbaha.113.302249
Subject(s) - vascular smooth muscle , cotransporter , phosphate , calcification , smooth muscle , chemistry , vascular disease , sodium , medicine , microbiology and biotechnology , biochemistry , anatomy , endocrinology , biology , organic chemistry
Elevated serum phosphate has emerged as a major risk factor for vascular calcification. The sodium-dependent phosphate cotransporter, PiT-1, was previously shown to be required for phosphate-induced osteogenic differentiation and calcification of cultured human vascular smooth muscle cells (VSMCs), but its importance in vascular calcification in vivo and the potential role of its homologue, PiT-2, have not been determined. We investigated the in vivo requirement for PiT-1 in vascular calcification using a mouse model of chronic kidney disease and the potential compensatory role of PiT-2 using in vitro knockdown and overexpression strategies.
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