2 H 2 O-Based High-Density Lipoprotein Turnover Method for the Assessment of Dynamic High-Density Lipoprotein Function in Mice | Zendy

Takhar Kasumov | Zendy; Belinda Willard | Zendy; Ling Li | Zendy; Min Li | Zendy; Heather Conger | Zendy; Jennifer A. Buffa | Zendy; Stephen F. Previs | Zendy; Arthur J. McCullough | Zendy; Stanley L. Hazen | Zendy; Jonathan D. Smith | Zendy

Open Access

² H ₂ O-Based High-Density Lipoprotein Turnover Method for the Assessment of Dynamic High-Density Lipoprotein Function in Mice

Author(s) -

Takhar Kasumov,

Belinda Willard,

Ling Li,

Min Li,

Heather Conger,

Jennifer A. Buffa,

Stephen F. Previs,

Arthur J. McCullough,

Stanley L. Hazen,

Jonathan D. Smith

Publication year - 2013

Publication title -

arteriosclerosis thrombosis and vascular biology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.007

H-Index - 270

eISSN - 1524-4636

pISSN - 1079-5642

DOI - 10.1161/atvbaha.113.301700

Subject(s) - function (biology) , lipoprotein , low density lipoprotein receptor related protein 8 , high density lipoprotein , chemistry , endocrinology , medicine , biochemistry , very low density lipoprotein , biology , cholesterol , microbiology and biotechnology

High-density lipoprotein (HDL) promotes reverse cholesterol transport from peripheral tissues to the liver for clearance. Reduced HDL-cholesterol (HDLc) is associated with atherosclerosis; however, as a predictor of cardiovascular disease, HDLc has limitations because it is not a direct marker of HDL functionality. Our objective was to develop a mass spectrometry-based method for the simultaneous measurement of HDLc and ApoAI kinetics in mice, using a single (2)H2O tracer, and use it to examine genetic and drug perturbations on HDL turnover in vivo.

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