Phosphorylation of G Protein–Coupled Receptor Kinase 2–Interacting Protein 1 Tyrosine 392 Is Required for Phospholipase C-γ Activation and Podosome Formation in Vascular Smooth Muscle Cells | Zendy

Jing Wang | Zendy; Guoyong Yin | Zendy; Prashanthi Me | Zendy; Jinjiang Pang | Zendy; Elaine M. Smolock | Zendy; Chen Yan | Zendy; Bradford C. Berk | Zendy

Open Access

Phosphorylation of G Protein–Coupled Receptor Kinase 2–Interacting Protein 1 Tyrosine 392 Is Required for Phospholipase C-γ Activation and Podosome Formation in Vascular Smooth Muscle Cells

Author(s) -

Jing Wang,

Guoyong Yin,

Prashanthi Me,

Jinjiang Pang,

Elaine M. Smolock,

Chen Yan,

Bradford C. Berk

Publication year - 2010

Publication title -

arteriosclerosis thrombosis and vascular biology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.007

H-Index - 270

eISSN - 1524-4636

pISSN - 1079-5642

DOI - 10.1161/atvbaha.110.212415

Subject(s) - phosphorylation , podosome , microbiology and biotechnology , receptor tyrosine kinase , proto oncogene tyrosine protein kinase src , tyrosine phosphorylation , chemistry , vascular smooth muscle , biochemistry , smooth muscle , biology , endocrinology , cell , cytoskeleton

Podosomes, which are actin-rich structures, contribute to cell motility, matrix remodeling, and tissue remodeling. We have shown that G protein-coupled receptor kinase 2-interacting protein 1 (GIT1) colocalizes with podosomes and is important in podosome formation in endothelial cells. Src stimulates GIT1 tyrosine phosphorylation, which is critical for phospholipase C-γ (PLCγ) activation. In this study, we identified specific GIT1 tyrosines required for PLCγ activation and podosome formation in vascular smooth muscle cells (VSMC).

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