Open AccessEndogenous KLF4 Expression in Human Fetal Endothelial Cells Allows for Reprogramming to Pluripotency With Just OCT3/4 and SOX2—Brief Report
Author(s) -
Pai-Jiun Ho,
MingShyen Yen,
Jhong-De Lin,
LanSun Chen,
Hsin-I Hu,
Chun-Kai Yeh,
Chiu-Ying Peng,
ChenYu Lin,
ShawFang Yet,
B. Linju Yen
Publication year - 2010
Publication title -
arteriosclerosis thrombosis and vascular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.007
H-Index - 270
eISSN - 1524-4636
pISSN - 1079-5642
DOI - 10.1161/atvbaha.110.206540
Subject(s) - sox2 , reprogramming , klf4 , induced pluripotent stem cell , biology , somatic cell , embryonic stem cell , microbiology and biotechnology , stem cell , endogeny , cancer research , genetics , cell , gene , endocrinology
The introduction of 4 transcription factors-c-MYC, OCT3/4, SOX2, and KLF4--can reprogram somatic cells back to pluripotency. However, some of the factors used are oncogenic, making therapeutic application unfeasible. Although the use of adult stem cells expressing high endogenous levels of some of these factors allows for reprogramming with fewer exogenous genes, such cells are rare and may have accumulated genetic mutations. Our goal was to reprogram human somatic cells without oncogenic factors. We found that high endogenous expression of KLF4 in human umbilical vein endothelial cells (HUVECs) allows for generation of induced pluripotent stem cells (iPSCs) with just 2 nononcogenic factors, OCT3/4 and SOX2.
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