Open AccessElectron Microscopy of Human Plasma Lipoprotein Separated by Ultracentrifugation
Author(s) -
Dietrich E. Beischer
Publication year - 1954
Publication title -
circulation research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.899
H-Index - 336
eISSN - 1524-4571
pISSN - 0009-7330
DOI - 10.1161/01.res.2.2.164
Subject(s) - osmium tetroxide , electron microscope , ultracentrifuge , chemistry , lipoprotein , molecule , negative stain , osmium , biophysics , chromatography , biochemistry , cholesterol , organic chemistry , biology , physics , optics , ruthenium , catalysis
The particle size distribution of the Sf 30 “class” of serum lipoprotein was determined by the electron microscope. Most of the lipoprotein molecules are not spherical but have the shape of ellipsoids of rotation with an axis ratio of 1.5. The molecules of a homogeneous flotation “class” show differences in the reactivity of their lipid moiety demonstrated by their staining properties with osmium tetroxide.
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