Open AccessInteraction of α9β1 Integrin With Thrombospondin-1 Promotes Angiogenesis
Author(s) -
Izabela Staniszewska,
Shachi Zaveri,
Luis Del Valle,
Isabela Batista Oliva,
Vicki L. Rothman,
Sidney Croul,
D. A. Roberts,
Deane F. Mosher,
George P. Tuszynski,
Cezary Marcinkiewicz
Publication year - 2007
Publication title -
circulation research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.899
H-Index - 336
eISSN - 1524-4571
pISSN - 0009-7330
DOI - 10.1161/01.res.0000266662.98355.66
Subject(s) - integrin , angiogenesis , disintegrin , microbiology and biotechnology , matrigel , thrombospondin , thrombospondin 1 , chorioallantoic membrane , matricellular protein , biology , endothelial stem cell , laminin , neovascularization , integrin, beta 6 , cell adhesion , chemistry , receptor , extracellular matrix , cancer research , cell , biochemistry , in vitro , metalloproteinase , matrix metalloproteinase
Thrombospondin-1 is a multifunctional protein interacting with several cell surface receptors including integrins. We found that it is a ligand for α9β1 integrin, and has an integrin binding site within its N-terminal domain (NoC1). Interaction of thrombospondin-1 and its recombinant NoC1 domain with α9β1 integrin was confirmed in ELISA and cell adhesion assays. Binding of NoC1 to cells expressing α9β1 integrin activated signaling proteins such as Erk1/2 and paxillin. Blocking of this integrin by monoclonal antibody and the met-leu-asp-disintegrin inhibited dermal human microvascular endothelial cell proliferation and NoC1-induced migration of these cells. Immunohistochemical studies revealed that α9β1 is expressed on microvascular endothelium in several organs including skin, lung, heart and brain. NoC1 induced neovascularization in an experimental quail chorioallantoic membrane system and Matrigel plug formation assay in mice. This proangiogenic activity of NoC1 in vivo was inhibited by α9β1 inhibitors. In summary, our results revealed that α9β1 integrin expressed on microvascular endothelial cells interacts with thrombospondin-1, and this interaction is involved in modulation of angiogenesis.