α-Thrombin Stimulates sis -Inducing Factor-A DNA Binding Activity in Rat Aortic Smooth Muscle Cells | Zendy

G. Jayarama Bhat | Zendy; S. Thomas Abraham | Zendy; Harold A. Singer | Zendy; Kenneth M. Baker | Zendy

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α-Thrombin Stimulates sis -Inducing Factor-A DNA Binding Activity in Rat Aortic Smooth Muscle Cells

Author(s) -

G. Jayarama Bhat,

S. Thomas Abraham,

Harold A. Singer,

Kenneth M. Baker

Publication year - 1997

Publication title -

hypertension

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.986

H-Index - 265

eISSN - 1524-4563

pISSN - 0194-911X

DOI - 10.1161/01.hyp.29.1.356

Subject(s) - thrombin , hirudin , cycloheximide , discovery and development of direct thrombin inhibitors , thrombomodulin , vascular smooth muscle , chemistry , microbiology and biotechnology , biology , medicine , endocrinology , platelet , protein biosynthesis , smooth muscle

Exposure of rat aortic vascular smooth muscle cells to alpha-thrombin resulted in the appearance of sis-inducing factor-A (SIF-A)-like DNA binding activity. This response to alpha-thrombin was delayed (detectable at 1 hour) compared with the rapid activation (15 to 30 minutes) by platelet-derived growth factor and the cytokine interleukin-6. alpha-Thrombin-induced SIF-A was sensitive to treatment with the tyrosine kinase inhibitor genistein. The thrombin inhibitor hirudin prevented the alpha-thrombin-mediated SIF-A induction. Cycloheximide had no effect on the ability of alpha-thrombin to induce SIF-A, suggesting that induction does not require new protein synthesis. alpha-Thrombin-induced SIF-A could be resolved into two additional subcomplexes termed SIF-A, and SIF-As. Antibodies against Stat3 reacted with alpha-thrombin-induced SIF-Af, suggesting that Stat3 or a related protein is present in this subcomplex. Induction of SIF-A DNA binding activity may contribute to alpha-thrombin-mediated cellular responses, including wound healing, cell proliferation, and inflammation in the vasculature.

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