Open AccessExpression of brain natriuretic peptide gene in human heart. Production in the ventricle.
Author(s) -
Kiminori Hosoda,
Kazumasa Nakao,
Masashi Mukoyama,
Yoshihiko Saito,
Michihisa Jougasaki,
Gotaro Shirakami,
Sachie Suga,
Yoshihiro Ogawa,
Hirofumi Yasue,
Hiroo Imura
Publication year - 1991
Publication title -
hypertension
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.986
H-Index - 265
eISSN - 1524-4563
pISSN - 0194-911X
DOI - 10.1161/01.hyp.17.6.1152
Subject(s) - ventricle , brain natriuretic peptide , medicine , atrial natriuretic peptide , natriuretic peptide , npr2 , npr1 , atrium (architecture) , endocrinology , northern blot , messenger rna , cardiology , chemistry , heart failure , gene , atrial fibrillation , biochemistry
To elucidate the expression of the brain natriuretic peptide gene in the human heart, we have measured brain natriuretic peptide mRNA levels in hearts using the Northern blot hybridization method. Brain natriuretic peptide mRNA was present with a size of approximately 0.9 kb in the ventricle as well as in the atrium. The brain natriuretic peptide mRNA level in the ventricle was 52% of that in the atrium, whereas the atrial natriuretic peptide mRNA level in the ventricle was approximately two orders of magnitude lower than that in the atrium. Taking atrial and ventricular weights into account, the total amount of brain natriuretic peptide mRNA in the ventricle represented 77% of that in the whole heart. These results demonstrate that most of brain natriuretic peptide mRNA occurs in the ventricle, in contrast with atrial natriuretic peptide mRNA, which is present mainly in the atrium, indicating that the ventricle is a major production site of brain natriuretic peptide.
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