Open AccessConverting enzyme activity and angiotensin metabolism in the dog brainstem.
Author(s) -
Robson A.S. Santos,
K. Bridget Brosnihan,
Mark C. Chappell,
Jorge L. Pesquero,
Cheryl L. Chernicky,
Lewis Joel Greene,
Carlos M. Ferrario
Publication year - 1988
Publication title -
hypertension
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.986
H-Index - 265
eISSN - 1524-4563
pISSN - 0194-911X
DOI - 10.1161/01.hyp.11.2_pt_2.i153
Subject(s) - brainstem , endocrinology , angiotensin ii , medicine , renin–angiotensin system , chemistry , norepinephrine , spinal cord , angiotensin converting enzyme , monoamine neurotransmitter , serotonin , metabolite , central nervous system , biology , dopamine , blood pressure , receptor , neuroscience
The concentrations of angiotensin converting enzyme (ACE) activity, norepinephrine, and serotonin were measured in microdissected regions of the dog's brainstem and spinal cord. In addition, we determined the in vitro metabolism of 125I-angiotensin I (Ang I) in homogenates of the same brain punch regions. High ACE-specific activity was found in the monoamine-containing regions of the brainstem and in the intermediolateral column of the spinal cord. In brainstem homogenates 125I-Ang I was metabolized to angiotensin II (Ang-[1-8]) and the N-terminal heptapeptide Ang-(1-7). In the presence of MK 422 (50 microM), Ang-(1-7) was still generated, while the production of Ang-(1-8) was inhibited. This study revealed the presence of high ACE activity in monoamine regions of dog brainstem and spinal cord, and showed that the metabolite Ang-(1-7) is the major product generated from Ang I in the presence and absence of ACE inhibition.
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