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Feeding chicks a mash enriched with 0.25 per cent cholesterol plus 5 per cent cottonseed oil induces a minimal sustained hypercholesterolemia. After 35 weeks of this regimen, birds exhibited a high incidence of gross cholesterol-induced atherosclerosis, particularly prominent in the thoracic aorta. The presence of atherosclerosis in these chicks with minimal diet-induced hypercholesterolemia lends further experimental support to the concept of the relationship of cholesterol in general, and exogenous cholesterol in particular, to the pathogenesis of human atherosclerosis.

Production of Experimental Cholesterol-Induced Atherosclerosis in Chicks with Minimal Hypercholesterolemia and Organ Lipidosis