Optimized Plasmid Construction Strategy for Cas9 | Zendy

Jianyong Xu | Zendy; Wenlei Li | Zendy; Md. Munnaf Hossen | Zendy; Yuning Jia | Zendy; Lingyun Li | Zendy; Zhong Huang | Zendy

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Optimized Plasmid Construction Strategy for Cas9

Author(s) -

Jianyong Xu,

Wenlei Li,

Md. Munnaf Hossen,

Yuning Jia,

Lingyun Li,

Zhong Huang

Publication year - 2018

Publication title -

cellular physiology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.486

H-Index - 87

eISSN - 1421-9778

pISSN - 1015-8987

DOI - 10.1159/000491669

Subject(s) - plasmid , guide rna , crispr , cas9 , genome editing , endonuclease , computational biology , nuclease , biology , rna , genome engineering , computer science , dna , genetics , gene

The target genome editing technology not only plays an important role in basic biology studies but also holds a great promise for potential clinical applications. The new generation of engineered nuclease RGEN (RNA Guided EndoNuclease) is much easier to construct and modify, and attracts more attentions. In the current study, we compared different plasmid construction strategies of Cas9-gRNA (guide RNA).

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