Open AccessExtended Donor Typing by Pooled Capillary Electrophoresis: Impact in a Routine Setting
Author(s) -
Franz F. Wagner,
Andrea Doescher,
Rita Bittner,
Thomas Müller
Publication year - 2018
Publication title -
transfusion medicine and hemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.971
H-Index - 39
eISSN - 1660-3818
pISSN - 1660-3796
DOI - 10.1159/000490155
Subject(s) - allele , typing , multiplex , microbiology and biotechnology , antigen , dna sequencer , capillary electrophoresis , biology , polymerase chain reaction , genetics , gene , chemistry
PCR with sequence-specific priming using allele-specific fluorescent primers and analysis on a capillary sequencer is a standard technique for DNA typing. We aimed to adapt this method for donor typing in a medium-throughput setting.
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