Engineering the Rapid Adenovirus Production and Amplification (RAPA) Cell Line to Expedite the Generation of Recombinant Adenoviruses | Zendy

Qiang Wei | Zendy; Jiaming Fan | Zendy; Junyi Liao | Zendy; Yulong Zou | Zendy; Dongzhe Song | Zendy; JianXiang Liu | Zendy; Jing Cui | Zendy; Feng Liu | Zendy; Chao Ma | Zendy; Xue Hu | Zendy; Li Li | Zendy; Yichun Yu | Zendy; Xiangyang Qu | Zendy; Liqun Chen | Zendy; Xinyi Yu | Zendy; Zhicai Zhang | Zendy; Zhao Chen | Zendy; Zongyue Zeng | Zendy; Ruyi Zhang | Zendy; Shujuan Yan | Zendy; Xingye Wu | Zendy; Yi Shu | Zendy; Russell R. Reid | Zendy; Michael J. Lee | Zendy; Jennifer Moritis Wolf | Zendy; TongChuan He | Zendy

Open Access

Engineering the Rapid Adenovirus Production and Amplification (RAPA) Cell Line to Expedite the Generation of Recombinant Adenoviruses

Author(s) -

Qiang Wei,

Jiaming Fan,

Junyi Liao,

Yulong Zou,

Dongzhe Song,

JianXiang Liu,

Jing Cui,

Feng Liu,

Chao Ma,

Xue Hu,

Li Li,

Yichun Yu,

Xiangyang Qu,

Liqun Chen,

Xinyi Yu,

Zhicai Zhang,

Zhao Chen,

Zongyue Zeng,

Ruyi Zhang,

Shujuan Yan,

Xingye Wu,

Yi Shu,

Russell R. Reid,

Michael J. Lee,

Jennifer Moritis Wolf,

TongChuan He

Publication year - 2017

Publication title -

cellular physiology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.486

H-Index - 87

eISSN - 1421-9778

pISSN - 1015-8987

DOI - 10.1159/000475909

Subject(s) - transfection , hek 293 cells , transgene , cell culture , biology , adenoviridae , recombinant dna , microbiology and biotechnology , genetic enhancement , gene delivery , luciferase , viral vector , titer , gene , virology , virus , genetics

While recombinant adenoviruses are among the most widely-used gene delivery vectors and usually propagated in HEK-293 cells, generating recombinant adenoviruses remains time-consuming and labor-intense. We sought to develop a rapid adenovirus production and amplification (RAPA) line by assessing human Ad5 genes (E1A, E1B19K/55K, pTP, DBP, and DNA Pol) and OCT1 for their contributions to adenovirus production.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research