Variability among TSH Measurements Can Be Reduced by Combining a Glycoengineered Calibrator to Epitope-Defined Immunoassays | Zendy

Sandrine Donadio-Andréi | Zendy; Karim Chikh | Zendy; Christine Heuclin | Zendy; E. Kuczewski | Zendy; Anne Charrié | Zendy; AnneSophie Gauchez | Zendy; Cathérine Ronin | Zendy

Open Access

Variability among TSH Measurements Can Be Reduced by Combining a Glycoengineered Calibrator to Epitope-Defined Immunoassays

Author(s) -

Sandrine Donadio-Andréi,

Karim Chikh,

Christine Heuclin,

E. Kuczewski,

Anne Charrié,

AnneSophie Gauchez,

Cathérine Ronin

Publication year - 2016

Publication title -

european thyroid journal

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.23

H-Index - 10

eISSN - 2235-0802

pISSN - 2235-0640

DOI - 10.1159/000449463

Subject(s) - epitope , medicine , glycosylation , chinese hamster ovary cell , hormone , endocrinology , computational biology , antibody , immunology , biology , biochemistry , receptor

Measuring protein markers with variable glycosylation, such as thyroid-stimulating hormone (TSH), with high accuracy is not an easy task. Despite highly sensitive third-generation tests, discrepancies among TSH assays still remain unsolved and are the focus of important standardization efforts. Earlier work from our group showed that a lack of similarity in epitope expression between standards and samples may account for discordant hormone measurements. In this study, we aimed at producing a glycoengineered TSH with serum-type glycosylation and compared its immunological behavior to that of the international standards.

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