Targeting Nuclear Receptors with Lentivirus-Delivered Small RNAs in Primary Human Hepatocytes | Zendy

Maria Thomas | Zendy; Jessica K. Rieger | Zendy; Benjamin A. Kandel | Zendy; Kathrin Klein | Zendy; Ulrich M. Zanger | Zendy

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Targeting Nuclear Receptors with Lentivirus-Delivered Small RNAs in Primary Human Hepatocytes

Author(s) -

Maria Thomas,

Jessica K. Rieger,

Benjamin A. Kandel,

Kathrin Klein,

Ulrich M. Zanger

Publication year - 2014

Publication title -

cellular physiology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.486

H-Index - 87

eISSN - 1421-9778

pISSN - 1015-8987

DOI - 10.1159/000362976

Subject(s) - rna interference , small hairpin rna , nuclear receptor , multiplicity of infection , biology , microrna , lentivirus , transduction (biophysics) , gene silencing , microbiology and biotechnology , gene knockdown , cell culture , virology , rna , virus , gene , genetics , transcription factor , biochemistry , viral disease

RNA interference (RNAi) has tremendous potential for investigating gene function and for developing new therapies. Primary human hepatocytes (PHH) are the "gold standard" for studying the regulation of hepatic metabolism in vitro. However, application of RNAi in PHH has some technical hurdles. The objective of this study was to develop effective and robust protocol for transduction of PHH with lentiviral vectors.

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