Secreted Clusterin Gene Silencing Enhances Chemosensitivity of A549 Cells to Cisplatin Through AKT and ERK1/2 Pathwaysin Vitro | Zendy

Biyuan Zhang | Zendy; Kejun Zhang | Zendy; Zimin Liu | Zendy; Fengyun Hao | Zendy; Mei Wang | Zendy; Xiangchun Li | Zendy; Zongsiu Yin | Zendy; Hui Liang | Zendy

Open Access

Secreted Clusterin Gene Silencing Enhances Chemosensitivity of A549 Cells to Cisplatin Through AKT and ERK1/2 Pathwaysin Vitro

Author(s) -

Biyuan Zhang,

Kejun Zhang,

Zimin Liu,

Fengyun Hao,

Mei Wang,

Xiangchun Li,

Zongsiu Yin,

Hui Liang

Publication year - 2014

Publication title -

cellular physiology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.486

H-Index - 87

eISSN - 1421-9778

pISSN - 1015-8987

DOI - 10.1159/000358685

Subject(s) - a549 cell , small hairpin rna , clusterin , cisplatin , gene knockdown , gene silencing , cancer research , transfection , apoptosis , chemistry , in vitro , microbiology and biotechnology , rna interference , rna , biology , gene , biochemistry , chemotherapy , genetics

Several studies have shown secreted clusterin (sCLU) silencing directed against sCLU mRNA in sCLU-rich lung cancer cell lines sensitized cells to chemotherapy. However, the molecular mechanisms underlying the effect of sCLU silencing on lung cancer cell chemosensitivity is not known. In the present study, we aimed to determine that vector expressing short hairpin RNA against sCLU RNA (sCLU-shRNA) enhances the chemosensitivity in human small cell lung cancer A549 cells in vitro by inhibition of phosphorylated ERK1/2 (p-ERK1/2) and Akt (p-Akt).

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