Analysis of Genomic DNA Methylation Levels in Human Placenta using Liquid Chromatography-Electrospray Ionization Tandem Mass Spectrometry | Zendy

Sulistyo Emantoko Dwi Putra | Zendy; Corineuber | Zendy; Christoph Reichetzeder | Zendy; Berthold Hocher | Zendy; Burkhard Kleuser | Zendy

Open Access

Analysis of Genomic DNA Methylation Levels in Human Placenta using Liquid Chromatography-Electrospray Ionization Tandem Mass Spectrometry

Author(s) -

Sulistyo Emantoko Dwi Putra,

Corineuber,

Christoph Reichetzeder,

Berthold Hocher,

Burkhard Kleuser

Publication year - 2014

Publication title -

cellular physiology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.486

H-Index - 87

eISSN - 1421-9778

pISSN - 1015-8987

DOI - 10.1159/000358666

Subject(s) - dna methylation , epigenetics , placenta , methylation , biology , dna , offspring , microbiology and biotechnology , andrology , gene , fetus , gene expression , pregnancy , genetics , medicine

DNA-methylation is a common epigenetic tool which plays a crucial role in gene regulation and is essential for cell differentiation and embryonic development. The placenta is an important organ where gene activity can be regulated by epigenetic DNA modifications, including DNA methylation. This is of interest as, the placenta is the interface between the fetus and its environment, the mother. Exposure to environmental toxins and nutrition during pregnancy may alter DNA methylation of the placenta and subsequently placental function and as a result the phenotype of the offspring. The aim of this study was to develop a reliable method to quantify DNA methylation in large clinical studies. This will be a tool to analyze the degree of DNA methylation in the human placenta in relationship to clinical readouts.

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