A Single-Tube Real-Time PCR Assay for Mycoplasma Detection as a Routine Quality Control of Cell Therapeutics | Zendy

Karin Janetzko | Zendy; Gabi Rink | Zendy; Andrea Hecker | Zendy; Karen Bieback | Zendy; Harald Klüter | Zendy; Peter Bugert | Zendy

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A Single-Tube Real-Time PCR Assay for Mycoplasma Detection as a Routine Quality Control of Cell Therapeutics

Author(s) -

Karin Janetzko,

Gabi Rink,

Andrea Hecker,

Karen Bieback,

Harald Klüter,

Peter Bugert

Publication year - 2013

Publication title -

transfusion medicine and hemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 39

eISSN - 1660-3818

pISSN - 1660-3796

DOI - 10.1159/000357096

Subject(s) - mollicutes , taqman , microbiology and biotechnology , serial dilution , polymerase chain reaction , real time polymerase chain reaction , biology , mycoplasmataceae , bacteria , dna , mycoplasma , medicine , genetics , gene , alternative medicine , pathology

Contamination of cell culture and biological material by mollicute species is an important safety issue and requires testing. We have developed a singletube real-time polymerase chain reaction (PCR) assay for rapid detection of Mollicutes species stipulated by the European Pharmacopeia.

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