Specific Nucleotide Changes in the Subgenomic Promoter Region Influence Infectivity of the Sindbis Virus | Zendy

Wuyang Zhu | Zendy; Ting Zhao | Zendy; Guodong Liang | Zendy

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Specific Nucleotide Changes in the Subgenomic Promoter Region Influence Infectivity of the Sindbis Virus

Author(s) -

Wuyang Zhu,

Ting Zhao,

Guodong Liang

Publication year - 2013

Publication title -

intervirology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.641

H-Index - 61

eISSN - 1423-0100

pISSN - 0300-5526

DOI - 10.1159/000351081

Subject(s) - subgenomic mrna , sindbis virus , biology , virology , alphavirus , nucleotide , virus , infectivity , transcription (linguistics) , recombinant dna , nucleic acid sequence , microbiology and biotechnology , gene , genetics , rna , linguistics , philosophy

Transcription of the subgenomic mRNA of Sindbis virus (SINV) is initiated at a subgenomic promoter (SP). Alignment of SINV sequences identified a 68-nucleotide conserved domain spanning -19 to +49 relative to the subgenomic mRNA start site. Nucleotide T or C is present at -18 or +49 in all known SINVs while a Sindbis-like virus XJ-160 has an A or T at a corresponding position. Our results indicate that deletion or substitution of the T at +49 decreased the activity of SP, while substituting T for A at -18 did not decrease the activity of SP or genetic stability of recombinant SINV.

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