Novel PCR Assay for Determining the Genetic Sex of Mice | Zendy

L. McFarlane | Zendy; Bá Vương Trương | Zendy; James S. Palmer | Zendy; Dagmar Wilhelm | Zendy

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Novel PCR Assay for Determining the Genetic Sex of Mice

Author(s) -

L. McFarlane,

Bá Vương Trương,

James S. Palmer,

Dagmar Wilhelm

Publication year - 2013

Publication title -

sexual development

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.528

H-Index - 44

eISSN - 1661-5433

pISSN - 1661-5425

DOI - 10.1159/000348677

Subject(s) - sexing , biology , primer (cosmetics) , amplicon , genetics , y chromosome , sexual differentiation , testis determining factor , mutant , polymerase chain reaction , microbiology and biotechnology , gene , chemistry , organic chemistry

A number of studies require the determination of the genetic sex of mouse embryos before sexual differentiation and/or of mutant mice that display partial or complete sex reversal. The majority of current methods for sexing by PCR involve multiplexing of 2 primer pairs. We have developed a novel sexing PCR using a single primer pair that amplifies fragments from the X and the Y chromosome with a clear size difference between the respective amplicons. This assay provides a rapid and reliable method to identify the genetic sex of mice across different mouse strains.

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