Dental Enamel Structure Is Altered by Expression of Dominant Negative RhoA in Ameloblasts | Zendy

Yong Li | Zendy; Megan K. Pugach | Zendy; Melissa A. Kuehl | Zendy; Li Peng | Zendy; Jessica Bouchard | Zendy; Soon Young Hwang | Zendy; Carolyn W. Gibson | Zendy

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Dental Enamel Structure Is Altered by Expression of Dominant Negative RhoA in Ameloblasts

Author(s) -

Yong Li,

Megan K. Pugach,

Melissa A. Kuehl,

Li Peng,

Jessica Bouchard,

Soon Young Hwang,

Carolyn W. Gibson

Publication year - 2011

Publication title -

cells tissues organs

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.662

H-Index - 82

ISSN - 1422-6405

DOI - 10.1159/000324559

Subject(s) - rhoa , ameloblast , enamel paint , chemistry , amelogenesis , microbiology and biotechnology , sodium fluoride , biology , fluoride , signal transduction , dentistry , medicine , inorganic chemistry

Using in vitrotooth germ cultures and analysis by confocal microscopy, ameloblasts treated with sodium fluoride were found to have elevated amounts of filamentous actin. Because this response is reduced by inhibitors of the Rho/ROCK signaling pathway, we generated mice that express dominant negative RhoA (RhoA(DN)) in ameloblasts for in vivo analysis. Expression of the EGFP-RhoA(DN) fusion protein was evaluated by RT-PCR and immunohistochemistry, and teeth were analyzed by scanning electron microscopy. The 3 strains expressed at either low (TgEGFP-RhoA(DN)-8), intermediate (TgEGFP-RhoA(DN)-2), or high (TgEGFP-RhoA(DN)-13) levels, and the molar teeth from the 3 strains had enamel hypoplasia and surface defects. We conclude that RhoA(DN) expressed in ameloblasts interferes with normal enamel development through the pathway that is induced by sodium fluoride.

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