Sensitivity of Chloride Efflux vs. Transepithelial Measurements in Mixed CF and Normal Airway Epithelial Cell Populations | Zendy

Beate Illek | Zendy; Dachuan Lei | Zendy; Horst Fischer | Zendy; Dieter C. Gruenert | Zendy

Open Access

Sensitivity of Chloride Efflux vs. Transepithelial Measurements in Mixed CF and Normal Airway Epithelial Cell Populations

Author(s) -

Beate Illek,

Dachuan Lei,

Horst Fischer,

Dieter C. Gruenert

Publication year - 2010

Publication title -

cellular physiology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.486

H-Index - 87

eISSN - 1421-9778

pISSN - 1015-8987

DOI - 10.1159/000324011

Subject(s) - ussing chamber , efflux , cystic fibrosis transmembrane conductance regulator , cystic fibrosis , transepithelial potential difference , population , biology , mucus , cell culture , epithelium , chloride channel , microbiology and biotechnology , biophysics , chemistry , ion transporter , in vitro , biochemistry , medicine , genetics , membrane , ecology , environmental health

While the Cl(-) efflux assays are relatively straightforward, their ability to assess the efficacy of phenotypic correction in cystic fibrosis (CF) tissue or cells may be limited. Accurate assessment of therapeutic efficacy, i.e., correlating wild type CF transmembrane conductance regulator (CFTR) levels with phenotypic correction in tissue or individual cells, requires a sensitive assay.

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