A Microsphere-Based Suspension Array for Blood Group Molecular Typing: An Update | Zendy

Francesca Drago | Zendy; Katerina Karpasitou | Zendy; Laura Spinardi | Zendy; Loretta Crespiatico | Zendy; M. Scalamogna | Zendy; Francesca Poli | Zendy

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A Microsphere-Based Suspension Array for Blood Group Molecular Typing: An Update

Author(s) -

Francesca Drago,

Katerina Karpasitou,

Laura Spinardi,

Loretta Crespiatico,

M. Scalamogna,

Francesca Poli

Publication year - 2010

Publication title -

transfusion medicine and hemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 39

eISSN - 1660-3818

pISSN - 1660-3796

DOI - 10.1159/000322039

Subject(s) - multiplex , genotyping , serology , typing , multiplex polymerase chain reaction , concordance , microbiology and biotechnology , genotype , microsphere , polymerase chain reaction , blood typing , biology , immunology , genetics , antibody , gene , chemical engineering , engineering

SUMMARY: BACKGROUND: In a previous publication we described a method for Jk(a)/Jk(b), Fy(a)/Fy(b), S/s, K/k, Kp(a)/Kp(b), Js(a)/Js(b), Co(a)/Co(b), and Lu(a)/Lu(b) genotyping based on a microsphere suspension array. Here, an improved version of the assay is presented. METHODS: TWO MULTIPLEX POLYMERASE CHAIN REACTIONS (PCR) WERE DEVELOPED: one for amplification of samples routinely tested and the other for those systems that are tested less frequently. Each biotinylated PCR product is hybridized in a single multiplex assay. A total of 2,020 samples were analyzed, and the genotypes were compared to the blood group phenotypes. RESULTS: There have been no discrepancies with the serology results other than null and/or weak phenotypes. CONCLUSION: In its present form, the method presented here has the capacity to genotype hundreds of a samples in few hours with a high concordance rate with serology.

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