Endogenous Control Genes in Prostate Cells: Evaluation of Gene Expression Using ‘Real-Time’ Quantitative Polymerase Chain Reaction
Author(s) -
Emmanuel Okechukwu Nna,
Ibtisam E. Tothill,
Linmarie Ludeman,
T. A. Bailey
Publication year - 2010
Publication title -
medical principles and practice
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.426
H-Index - 45
eISSN - 1423-0151
pISSN - 1011-7571
DOI - 10.1159/000320300
Subject(s) - prostate , prostate cancer , medicine , real time polymerase chain reaction , pca3 , andrology , microbiology and biotechnology , prostate specific antigen , gene , gene expression , rectal examination , biology , cancer , biochemistry
Our aims were to measure the level of expression of Abelson (ABL1), β-glucuronidase (GUS) and glucose-6-phosphate dehydrogenase (G6PD) genes in exfoliated urine cells from healthy and transrectal ultrasound biopsy patients with elevated prostate-specific antigen levels and/or abnormal digital rectal examinations or urinary symptoms indicative of prostate problems, as well as in archived formalin-fixed paraffin-embedded prostate materials.
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