HPV-58 Molecular Variants Exhibit Different Transcriptional Activity | Zendy

Tainá Raiol | Zendy; Regina Maria Santos de Amorim | Zendy; Pedro A. F. Galante | Zendy; Cláudia Renata Fernandes Martins | Zendy; Luisa L. Villa | Zendy; Laura Sichero | Zendy

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HPV-58 Molecular Variants Exhibit Different Transcriptional Activity

Author(s) -

Tainá Raiol,

Regina Maria Santos de Amorim,

Pedro A. F. Galante,

Cláudia Renata Fernandes Martins,

Luisa L. Villa,

Laura Sichero

Publication year - 2010

Publication title -

intervirology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.641

H-Index - 61

eISSN - 1423-0100

pISSN - 0300-5526

DOI - 10.1159/000320199

Subject(s) - transfection , luciferase , promoter activity , biology , transcription (linguistics) , microbiology and biotechnology , transcriptional activity , mutation , human papillomavirus , gene , transcription factor , virology , promoter , genetics , gene expression , medicine , linguistics , philosophy

Early promoter activity of HPV-58 molecular variants isolated from high-grade cervical lesions in Brazil was compared. Luciferase reporter assays were conducted in C33 cells transfected with the complete long control region of 3 molecular variants of HPV-58 as well as HPV-58, -18 or -16 prototypes. The HPV-58 prototype and Bsb-329 and Bsb-367 variants showed a promoter activity similar to that of HPV-16, but lower than that of Bsb-295 and HPV-18. The introduction of the Bsb-295 7788 mutation into the HPV-58 prototype resulted in the enhancement of transcription closer to Bsb-295 and HPV-18. These results could impact the expression of E6 and E7 viral oncogenes.

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