Variability of Two Plaque Sampling Methods in Quantitation of <i>Streptococcus mutans</i>
Author(s) -
Kuei-Ling Hsu,
Robert Osgood,
Gary Cutter,
Noel K. Childers
Publication year - 2010
Publication title -
caries research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.355
H-Index - 100
eISSN - 1421-976X
pISSN - 0008-6568
DOI - 10.1159/000314066
Subject(s) - reproducibility , streptococcus mutans , dental plaque , molar , significant difference , sampling (signal processing) , dentistry , mean difference , chemistry , medicine , chromatography , confidence interval , biology , bacteria , genetics , filter (signal processing) , computer science , computer vision
The aim of this study was to determine the reproducibility of individual versus pooled plaque sampling of permanent first molars (PFM) to quantitate Streptococcus mutans (SM)/total streptococci (TS). Ten individual and pooled plaque samples were collected from 35 subjects, randomly assigned to individual-first or pooled-first group. Plaque samples were processed and quantified for SM and TS. SM/TS ratio was used to determine the reproducibility within two group samples. Mean percentage of SM/TS in both methods were not significantly different. However, within subject detection of SM was found to be significantly more sensitive for individual sampling method. Despite the lack of a difference between both methods for SM/TS quantitation, the difference in SM detection suggests that individual sampling is more sensitive.
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