Validation of Endogenous Internal Real-Time PCR Controls in Renal Tissues | Zendy

Xiangqin Cui | Zendy; Juling Zhou | Zendy; Jing Qiu | Zendy; Martin R. Johnson | Zendy; Michal Mrug | Zendy

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Validation of Endogenous Internal Real-Time PCR Controls in Renal Tissues

Author(s) -

Xiangqin Cui,

Juling Zhou,

Jing Qiu,

Martin R. Johnson,

Michal Mrug

Publication year - 2009

Publication title -

american journal of nephrology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.394

H-Index - 85

eISSN - 1421-9670

pISSN - 0250-8095

DOI - 10.1159/000235993

Subject(s) - housekeeping gene , taqman , real time polymerase chain reaction , kidney , endogeny , medicine , gene expression , reference genes , gene chip analysis , gene , kidney disease , biology , microarray , genetics

Endogenous internal controls ('reference' or 'housekeeping' genes) are widely used in real-time PCR (RT-PCR) analyses. Their use relies on the premise of consistently stable expression across studied experimental conditions. Unfortunately, none of these controls fulfills this premise across a wide range of experimental conditions; consequently, none of them can be recommended for universal use.

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