A GTP Analogue Induces Calcium Release but Not Secretion in Rat Mast Cells | Zendy

Friederike von zur Mühlen | Zendy; Reinhold Penner | Zendy

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A GTP Analogue Induces Calcium Release but Not Secretion in Rat Mast Cells

Author(s) -

Friederike von zur Mühlen,

Reinhold Penner

Publication year - 1991

Publication title -

international archives of allergy and immunology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.696

H-Index - 100

eISSN - 1423-0097

pISSN - 1018-2438

DOI - 10.1159/000235329

Subject(s) - secretion , mast cell , calcium , mast (botany) , immunology , gtp' , chemistry , biology , medicine , microbiology and biotechnology , endocrinology , biochemistry , enzyme

Two G protein-mediated events, calcium release and secretion, were measured in single rat peritoneal mast cells using the patch-clamp technique. Various phosphorothioate analogues of GTP were introduced into the cells. While GTP gamma S and Rp-GTP alpha S activated both processes, Rp-GTP beta S was found to induce repetitive calcium release in the absence of exocytosis. This response was modulated by IP3, heparin, compound 48/80, and ATP. Our results suggest that Rp-GTP beta S simulates Gp, which links receptors to calcium release.

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