His6-Tagged UL35 Protein of Duck Plague Virus: Expression, Purification, and Production of Polyclonal Antibody | Zendy

Mingsheng Cai | Zendy; Anchun Cheng | Zendy; Mingshu Wang | Zendy; Li-Chan Zhao | Zendy; Dekang Zhu | Zendy; Qihui Luo | Zendy; Fei Liu | Zendy; Xiaoyue Chen | Zendy

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His6-Tagged UL35 Protein of Duck Plague Virus: Expression, Purification, and Production of Polyclonal Antibody

Author(s) -

Mingsheng Cai,

Anchun Cheng,

Mingshu Wang,

Li-Chan Zhao,

Dekang Zhu,

Qihui Luo,

Fei Liu,

Xiaoyue Chen

Publication year - 2009

Publication title -

intervirology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.641

H-Index - 61

eISSN - 1423-0100

pISSN - 0300-5526

DOI - 10.1159/000221833

Subject(s) - fusion protein , microbiology and biotechnology , recombinant dna , polyclonal antibodies , capsid , inclusion bodies , expression vector , affinity chromatography , biology , virus , escherichia coli , virology , gene , chemistry , antibody , biochemistry , enzyme , immunology

Duck plague virus (DPV), the causative agent of duck plague (DP), is an alphaherpesvirus that causes an acute, febrile, contagious, and septic disease of waterfowl. UL35 protein (VP26) is a major capsid protein encoded by the UL35 gene, which is located in the unique long (UL) region of the DPV genome. To investigate the specific roles of VP26, the UL35 gene was amplified from the DPV DNA by polymerase chain reaction (PCR) and subcloned into pET-32a(+).

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