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Whole-Blood Polymerase Chain Reaction and Restriction Fragment Length Polymorphism: A Simplified Method by Microwave Irradiation
Author(s) -
Mehrez M. Jadaon,
Ali A. Dashti,
Hend L. Lewis,
Fatema M. Habeeb
Publication year - 2009
Publication title -
medical principles and practice
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.426
H-Index - 45
eISSN - 1423-0151
pISSN - 1011-7571
DOI - 10.1159/000215724
Subject(s) - restriction fragment length polymorphism , polymerase chain reaction , microbiology and biotechnology , dna extraction , dna , chromatography , biology , chemistry , genetics , gene
The aim of the present study was to develop a simple, quick and cheap method to process whole-blood samples for the molecular techniques polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) without the use of expensive reagents or sophisticated machines.

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