Antihypoxic Effects of Neuroglobin in Hypoxia-Preconditioned Mice and SH-SY5Y Cells | Zendy

Guo Shao | Zendy; Kerui Gong | Zendy; Jiang Li | Zendy; Xijin Xu | Zendy; Cui-Ying Gao | Zendy; Xian-Zhi Zeng | Zendy; Guowei Lu | Zendy; Xia Huo | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Antihypoxic Effects of Neuroglobin in Hypoxia-Preconditioned Mice and SH-SY5Y Cells

Author(s) -

Guo Shao,

Kerui Gong,

Jiang Li,

Xijin Xu,

Cui-Ying Gao,

Xian-Zhi Zeng,

Guowei Lu,

Xia Huo

Publication year - 2009

Publication title -

neurosignals

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.755

H-Index - 67

eISSN - 1424-8638

pISSN - 1424-862X

DOI - 10.1159/000209867

Subject(s) - neuroglobin , sh sy5y , hypoxia (environmental) , neuroprotection , blot , in vitro , microbiology and biotechnology , messenger rna , downregulation and upregulation , in vivo , transfection , biology , chemistry , pharmacology , cell culture , biochemistry , globin , gene , genetics , organic chemistry , oxygen , neuroblastoma

This work aims at investigating the neuroprotective effects of neuroglobin (Ngb) in vivo and in vitro. RT-PCR and Western blotting were used to examine Ngb mRNA and protein levels in the mouse cortex after acute and repeated exposure to hypoxia. The cDNAs of mouse Ngb were cloned and transfected into SH-SY5Y cells to examine Ngb function in vitro. Expression of Ngb and mRNA was upregulated in the cortex of mice preconditioned by repetitive exposure to hypoxia. Tolerance to hypoxia of Ngb-transformed SH-SY5Y cells was enhanced. These results suggest that Ngb might be involved in hypoxic preconditioning which protects neurons from hypoxic injury.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research