Mineralization and Expression of Col1a1-3.6GFP Transgene in Primary Dental Pulp Culture | Zendy

Anamaria Balic | Zendy; Barbara Rodgers | Zendy; Mina Mina | Zendy

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Mineralization and Expression of Col1a1-3.6GFP Transgene in Primary Dental Pulp Culture

Author(s) -

Anamaria Balic,

Barbara Rodgers,

Mina Mina

Publication year - 2008

Publication title -

cells tissues organs

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.662

H-Index - 82

ISSN - 1422-6405

DOI - 10.1159/000154813

Subject(s) - mineralization (soil science) , ascorbic acid , chemistry , dentin , pulp (tooth) , cell culture , biochemistry , dexamethasone , microbiology and biotechnology , dentistry , biology , endocrinology , food science , medicine , organic chemistry , genetics , nitrogen

We have examined and compared the effects of various differentiation-inducing media on mineralization, cell morphology and expression of pOBCol3.6GFP (3.6-GFP) in primary dental pulp cultures derived from 3.6-GFP transgenic mice. Our results show that media containing ascorbic acid only could not induce mineralization in primary dental pulp cultures. On the other hand, media containing ascorbic acid and beta-glycerophosphate induced formation of mineralized matrix-containing dentin. The amount of mineralized matrix was increased by addition of dexamethasone. Cells treated with ascorbic acid and beta-glycerophosphate were fibroblast like and cells treated with dexamethasone were cuboidal. In all culture conditions, high levels of 3.6-GFP were expressed in areas of mineralization.

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