English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

The flow cytometric enumeration of CD34+ cells, comprising both haematopoietic stem (HSC) and progenitor cells (HPC) is currently widely used to assess suitability of stem cell transplants for transplantation. However, to ensure the long-term reconstruction in human recipients, the enumeration specifically of those HSC possessing self-renewing and differentiation potential would be preferable. In the present study we addressed a multiparametric flow cytometric profiling of haematopoietic stem cells of umbilical cord (CB) and peripheral blood (PB) using stem cells markers (epitope- specific CD34, CD133, rhodamine-123 (Rho123) efflux, p-glycoprotein) to assess their stem cell features. In contrast to CB, the CD34 class III compartment of PB did not include all CD34 class I- and class II-expressing cells. In CB and PB, there was no clear correlation between CD34 epitope and CD133 expression. Furthermore, we detected CD133+ CD34- cells (0.07% of mononuclear cells) in CB. The functional ability to exclude Rho123 was mainly restricted to CD34- and CD133-co-expressing cells. However, CB cells showed a higher activity. Functionally active cells were characterised by their expression of the verapamil-sensitive protein pump p-glycoprotein 170 using the monoclonal antibody MRK16. It is therefore possible to use multiparametric flow cytometry to estimate the numbers of immature stem cells as CD34+ CD133+ (Rhodull) MRK16+. This would allow a more precise assessment of transplant quality, especially with respect to their long-term engraftment potential, and supports haemotherapy of malignant diseases.

transfusion medicine and hemotherapy

Flow Cytometric Immunophenotyping of Umbilical Cord and Peripheral Blood Haematopoietic Progenitor Cells by Different CD34 Epitopes, CD133, P-Glycoprotein Expression and Rhodamine-123 Efflux