In vitro Interconversion of the Soluble and Membrane- Integrated Forms of the Escherichia coli Glucose Enzyme II of the Phosphoenolpyruvate-Dependent Sugar-Transporting Phosphotransferase System | Zendy

Mohammad Aboulwafa | Zendy; Milton H. Saier | Zendy

Open Access

In vitro Interconversion of the Soluble and Membrane- Integrated Forms of the <i>Escherichia coli </i>Glucose Enzyme II of the Phosphoenolpyruvate-Dependent Sugar-Transporting Phosphotransferase System

Author(s) -

Mohammad Aboulwafa,

Milton H. Saier

Publication year - 2007

Publication title -

microbial physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.404

H-Index - 79

eISSN - 2673-1673

pISSN - 2673-1665

DOI - 10.1159/000099647

Subject(s) - pep group translocation , chemistry , permease , escherichia coli , biochemistry , phosphotransferase , phosphoenolpyruvate carboxykinase , membrane , enzyme , sugar , phospholipid , chromatography , gene

In previous publications, we have shown that integral membrane sugar permeases of the bacterial phosphotransferase system can exist in a 'soluble' (probably micellar) monomeric form (SII) as well as a membrane-integrated dimeric form (MII). We here show that the two forms of the his-tagged glucose permease of Escherichia coli can be interconverted in vitro. Conversion of MII to SII is promoted by (1) low protein concentration, (2) detergent, (3) high pH, and (4) phospholipase A(2) treatment. Conversion of SII to MII is promoted by: (1) high protein concentration, (2) adherence to and elution from an Ni(2+) column, (3) neutral pH, and (4) incorporation into phospholipid liposomes.

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