Mu-Opioid Receptor-Mediated Phosphorylation of IκB Kinase in Human Neuroblastoma SH-SY5Y Cells | Zendy

Andrew M.F. Liu | Zendy; Yung Hou Wong | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Mu-Opioid Receptor-Mediated Phosphorylation of IκB Kinase in Human Neuroblastoma SH-SY5Y Cells

Author(s) -

Andrew M.F. Liu,

Yung Hou Wong

Publication year - 2005

Publication title -

neurosignals

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.755

H-Index - 67

eISSN - 1424-8638

pISSN - 1424-862X

DOI - 10.1159/000086296

Subject(s) - iκb kinase , tank binding kinase 1 , microbiology and biotechnology , kinase , chemistry , sgk1 , map kinase kinase kinase , signal transduction , cyclin dependent kinase 9 , mitogen activated protein kinase kinase , biology , cancer research , protein kinase c , nf κb

Opioid receptors are involved in regulating neuronal survival. Here we demonstrate that activation of the mu-opioid receptor in human neuroblastoma SH-SY5Y cells led to the phosphorylations of IkappaB kinase (IKK) and p65, denoting the stimulation of the nuclear factor-kappaB (NFkappaB) transcription factor. This response was mediated through pertussis toxin-sensitive G proteins. The mu-opioid-induced IKK phosphorylation required extracellular signal-regulated protein kinase, phosphatidylinositol 3-kinase and c-Src. Moreover, c-Jun N-terminal kinase and calmodulin-dependent kinase II also participated in the IKK activation, despite the lack of involvement of phospholipase Cbeta and protein kinase C. These data suggest that the mu-opioid receptor is capable of simulating NFkappaB signaling via the phosphorylation of IKK and p65 in human neuroblastoma SH-SY5Y cells.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research