Assignment of the <i>MRPS35</i> gene to bovine chromosome 5q3.2→q3.4 by fluorescence in situ hybridization and confirmation by radiation hybrid mapping

The mitochondrial ribosomal protein S35 (MRPS35) belongs to a group of 15 proteins present in mammalian mitochondrial 28S subunits (MRPS22 through MRPS36) which are specific to mitochondrial ribosomes and help in protein synthesis within the mitochondrion (Cavdar Koc et al., 2001). Proteins in this group have no apparent homologs in bacterial, chloroplast, archaebacterial, or cytosolic ribosomes. The human MRPS35 gene consists of 8 exons spanning about 45.5 kb and was mapped on chromosome HSA12p11, inferred following radiation hybrid (RH) mapping (Kenmochi et al., 2001; NCBI map viewer, human genome build 34.3). Pseudogenes corresponding to this gene are found on HSA3p, 5q, and 10q (Zhang and Gerstein, 2003). The murine Mrps35 gene also consists of 8 exons and is located on mouse chromosome MMU6G3 (NCBI map viewer, mouse genome build 32). Because of the possible involvement of mitochondrial ribosomal defects in human diseases we have started to characterize the MRPS35 gene in cattle. This mapping report provides a basis for studying possible roles of MRPS35 defects in bovine mitochondrial disorders. We report here the assignment of the bovine MRPS35 gene to BTA5q3.2→q3.4 by fluorescence in situ hybridization (FISH) and RH mapping. This location is consistent with known conservation of synteny between cattle, human and mouse genomes (e.g. Hayes et al., 2003).