In vivo Confocal Laser Scanning Microscopy and Micropuncture in Intact Rat
Author(s) -
Yoshio Ohno,
Henrik Birn,
Erik Christensen
Publication year - 2005
Publication title -
nephron experimental nephrology
Language(s) - English
Resource type - Journals
ISSN - 1660-2129
DOI - 10.1159/000081794
Subject(s) - in vivo , confocal microscopy , confocal , endocytosis , fluorescence microscope , vesicle , confocal laser scanning microscopy , microscopy , kidney , proximal tubule , chemistry , transferrin , biophysics , microbiology and biotechnology , biology , pathology , fluorescence , biochemistry , receptor , medicine , endocrinology , optics , physics , membrane
Intravital microscopy theoretically provides the optimal conditions for studying specific organ functions. However, the application of microscopy in intact organs in vivo has been limited so far due to technical difficulties. The purpose of this study was to establish a method of in vivo confocal laser scanning microscopy (CLSM) for the study of endocytosis in proximal tubules of intact kidney.
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