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The 26S proteasome is a multicatalytic enzyme complex that plays a central role in all regulatory pathways such as cell cycle regulation, differentiation, and apoptosis. The 26S proteasome has three components, 20S proteasome, PA28 (Proteasome Activator 28) and PA700 (Proteasome Activator 700) subunits. The combination of PA28 and 20S proteasomes greatly increases the proteasome-mediated proteolysis activity, which is important in the processing and presentation of some antigens. The three genes related to PA28 and seven genes related to PA700 have been mapped by our group (Wang et al., 2003, 2004; Yu et al., 2004). Here we report our mapping results on the PSMA5, PSMB3 and PSMB6 genes, which were thought to be part of the 20S proteasome, the core of the 26S proteasome. Materials and methods

Mapping of three porcine 20S proteasome genes using the IMpRH panel