Expression and Activity of Cyclin-Dependent Kinases and Glycogen Synthase Kinase-3 during NT2 Neuronal Differentiation | Zendy

Marie Gompel | Zendy; Cathia Soulié | Zendy; Irène Ceballos-Picot | Zendy; Laurent Meijer | Zendy

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Expression and Activity of Cyclin-Dependent Kinases and Glycogen Synthase Kinase-3 during NT2 Neuronal Differentiation

Author(s) -

Marie Gompel,

Cathia Soulié,

Irène Ceballos-Picot,

Laurent Meijer

Publication year - 2004

Publication title -

neurosignals

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.755

H-Index - 67

eISSN - 1424-8638

pISSN - 1424-862X

DOI - 10.1159/000076567

Subject(s) - cyclin dependent kinase , cyclin dependent kinase 5 , kinase , microbiology and biotechnology , gsk 3 , cellular differentiation , biology , retinoic acid , glycogen synthase , cyclin dependent kinase 2 , protein kinase a , chemistry , cell cycle , biochemistry , phosphorylation , cell , gene

In the presence of retinoic acid undifferentiated NT2 cells turn into terminally differentiated hNT (or NT2N) neurons within 5 weeks. We have used this in vitro cellular model to investigate the changes in expression and activity of cyclin-dependent kinases (CDKs) and glycogen synthase kinase-3 (GSK-3) during this neuronal differentiation process. We here show that CDK1/2 protein level and kinase activity sharply decrease during the NT2-->hNT transition. In contrast, the activity of CDK5/p35 dramatically increases, probably as a result of an enhanced expression of p35 in a stable CDK5 level background. GSK-3 activity increases modestly during the differentiation of hNT cells, and this event correlates with enhanced expression of each of the three GSK-3 isoforms. Pharmacological inhibitors of CDKs and GSK-3 lead to a dose-dependent decrease in cell viability.

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