Detection of mRNA for Alpha-3 Chain of Type IV Collagen in the Glomerular Epithelium, and the Effect of Perfused Elastase on Its Expression | Zendy

Shoji Nogae | Zendy; Mari Michimata | Zendy; Tsutomu Araki | Zendy; Michiko Suzuki | Zendy; Itsuro Kazama | Zendy; Sadayoshi Ito | Zendy; Yutaka Imai | Zendy; Mitsunobu Matsubara | Zendy

Open Access

Detection of mRNA for Alpha-3 Chain of Type IV Collagen in the Glomerular Epithelium, and the Effect of Perfused Elastase on Its Expression

Author(s) -

Shoji Nogae,

Mari Michimata,

Tsutomu Araki,

Michiko Suzuki,

Itsuro Kazama,

Sadayoshi Ito,

Yutaka Imai,

Mitsunobu Matsubara

Publication year - 2002

Publication title -

the nephron journals/nephron journals

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.951

H-Index - 72

eISSN - 2235-3186

pISSN - 1660-8151

DOI - 10.1159/000065440

Subject(s) - in situ hybridization , elastase , renal cortex , messenger rna , kidney , microbiology and biotechnology , medicine , basement membrane , endocrinology , perfusion , epithelium , chemistry , biology , pathology , biochemistry , enzyme , gene

The type IV collagen is a complex of trimetric molecule composed of six genetically distinct polypeptide chains; alpha1-6(IV). Since alpha3(IV) distribute specifically in the glomerular basement membrane (GBM) of glomerular capillary, we tried to develop the detection methods for the transcripts of alpha3(IV) in glomerular epithelial cells (GEC) which produce most of the components for GBM. Then, using these molecular techniques, the influence of elastase, one of the proteases released from activated polymorphonuclear leukocytes at the site of inflammation, on GEC was determined as manifested by expressional alteration of alpha3(IV) mRNA.

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