Identification of Anticoagulant Activities in Salivary Gland Extracts of Four Horsefly Species (Diptera, Tabanidae) | Zendy

Mária Kazimírová | Zendy; M. Šulanová | Zendy; Milan Kozánek | Zendy; Peter Takáč | Zendy; Milan Labuda | Zendy; Adriana Patrícia | Zendy

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Identification of Anticoagulant Activities in Salivary Gland Extracts of Four Horsefly Species (Diptera, Tabanidae)

Author(s) -

Mária Kazimírová,

M. Šulanová,

Milan Kozánek,

Peter Takáč,

Milan Labuda,

Adriana Patrícia

Publication year - 2001

Publication title -

pathophysiology of haemostasis and thrombosis

Language(s) - English

Resource type - Journals

eISSN - 1424-8840

pISSN - 1424-8832

DOI - 10.1159/000048076

Subject(s) - chromogenic , coagulation , salivary gland , anticoagulant , chemistry , thrombin , chromatography , clotting time , biochemistry , biology , medicine , immunology , platelet

Anticoagulant activities against the extrinsic and intrinsic coagulation pathways were identified in salivary gland extracts (SGE) prepared from four tabanids (Hybomitra muehlfeldi, Tabanus autumnalis, Haematopota pluvialis, Heptatoma pellucens). All extracts prolonged human plasma clotting time in a dose-dependent manner and inhibited thrombin activity in the chromogenic substrate assay. Horsefly SGE did not inhibit factor Xa. Partial purification of SGE proteins using reversed-phase high-performance liquid chromatography revealed species-specific differences in the elution profiles and range of fractions with anticoagulant activities.

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