Open AccessTransplantation Strategies to Reconstruct the Injured Spinal Cord and its Peripheral Motor Connections in the Adult Rat
Author(s) -
Jean-Claude Horvat,
M Pécot-Dechavassine,
C. BailletDerbin,
JeanClaude Mira,
Jian Hui Ye,
Fatiha Rhrich,
Fatima Affane
Publication year - 1992
Publication title -
neural plasticity
Language(s) - English
Resource type - Journals
eISSN - 2090-5904
pISSN - 1687-5443
DOI - 10.1155/np.1992.261
Subject(s) - spinal cord , peripheral , medicine , spinal cord injury , transplantation , horseradish peroxidase , lesion , anatomy , peripheral nerve , neuroscience , surgery , biology , biochemistry , psychiatry , enzyme
We are currently working, in the adult rat, on two main experimental models of spinal injury (with special reference to peripheral motor connectivity) and its subsequent repair with the help ofPNS and/or CNS transplants. A first model involved a limited spinal lesion (determined by the insertion of one end of a 30 mm segment of a peripheral nerve autograft (PNG) into the right side of the cervical enlargement of the spinal cord) causing no apparent functional deficit. The other extremity of the PNG was inserted into a normally aneural area of a nearby skeletal muscle of the dorsal musculature which was carefully denervated prior to grafting. Two to 21 months following surgery, it was noticed, in the anaesthetized animal, that the reconnected muscle contracted under an adequate stimulation of the nerve bridge. Application of horseradish peroxidase (HRP) to the PNG led to an extensive neuronal labelling in
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