Modification of Cɛ mRNA Expression by EBV‐Encoded Latent Membrane Protein 1 | Zendy

Kazuhiko Hanashiro | Zendy; Shigeto Ohta | Zendy; Masanori Sunagawa | Zendy; Mariko Nakamura | Zendy; Mikio Suzuki | Zendy; Tadayoshi Kosugi | Zendy

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Modification of Cɛ mRNA Expression by EBV‐Encoded Latent Membrane Protein 1

Author(s) -

Kazuhiko Hanashiro,

Shigeto Ohta,

Masanori Sunagawa,

Mariko Nakamura,

Mikio Suzuki,

Tadayoshi Kosugi

Publication year - 2006

Publication title -

mediators of inflammation

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.37

H-Index - 97

eISSN - 1466-1861

pISSN - 0962-9351

DOI - 10.1155/mi/2006/68069

Subject(s) - messenger rna , gene expression , protein expression , biology , microbiology and biotechnology , virology , gene , genetics

To evaluate the effect of expression of latent membrane protein (LMP) 1 encoded byEpstein-Barr virus (EBV) on Cɛ mRNA expression, mRNA levels were examined by RT-PCRor Northern blot analysis upon transient transfection of LMP1 in the splenocytes derived fromBrown-Norway rats with or without immunization with 2,4-dinitrophenyl-conjugated Ascarissuum antigen. Splenocytes were transfected with LMP1 expression vector, pSG5-LMP1,using lipofection method. Cɛ mRNA levels were considerably increased by transfection with pSG5-LMP1 in the splenocytes derived from the nonimmunized rats; however, Cɛ mRNA levels were decreased in the splenocytes derived from the immunized rats. Cɛ mRNA expression in IgE-producing cells are modulated by LMP1, which might depend onthe differentiation status of B cells upon exposure to allergen

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