Simultaneous analysis of cytokines and co‐stimulatory molecules concentrations by ELISA technique andof probabilities of measurable concentrations of interleukins IL‐2, IL‐4, IL‐5, IL‐6, CXCL8 (IL‐8), IL‐10, IL‐13 occurring in plasma of healthy blood donors

Background. The cytokine network remarkably influenceshomeostasis. There is not a complex analysis describingphysiological values of soluble cytokines in healthy individuals.Methods. Authors used the ELISA method to detect solublecytokines and costimulatory molecules simultaneously in theplasma of 30 blood donors. Results. Data are shown as (arange of concentrations; median) IL-2 (0–74.48 pg/mL; 0),IL-4 (0–65.18 pg/mL; 0), IL-5 (0–86.69 pg/mL; 0),IL-6 (0–73.17 pg/mL; 0), IL-8 (0–74.8 pg/mL; 0),IL-10 (0–12.6 pg/mL; 0), IL-13 (0–3.9 pg/mL; 0),sCD40L (0–3.5 ng/mL; 0), and TNF-α (0–67.6; 0).For the IL-6 receptor, the concentration was 75.89±35.83 ng/mL (average ± SD), sCD23 28.9±15.22 ng/mL, and TGFβ1 14.98±12.39 ng/mL. Aspecial approach was taken for the analysis of values, which hadmedian 0. A model for the probability of detectable level'soccurrence for each interleukin was formulated with intervals ofconfidence. Conclusion. These results provide referencevalues for cytokine levels in the plasma of healthy individuals