Essential Notes Regarding the Design of Functional siRNAs for Efficient Mammalian RNAi | Zendy

Kumiko UiTei | Zendy; Yuki Naito | Zendy; Kaoru Saigo | Zendy

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Essential Notes Regarding the Design of Functional siRNAs for Efficient Mammalian RNAi

Author(s) -

Kumiko UiTei,

Yuki Naito,

Kaoru Saigo

Publication year - 2006

Publication title -

biomed research international

Language(s) - English

Resource type - Journals

eISSN - 2314-6141

pISSN - 2314-6133

DOI - 10.1155/jbb/2006/65052

Subject(s) - rna interference , small interfering rna , functional genomics , computational biology , trans acting sirna , biology , gene , genetics , rna , genomics , genome

Short interfering RNAs (siRNAs) are widely used to bring about RNAinterference (RNAi) in mammalian cells. Numerous siRNAs may bedesigned for any target gene though most of which would beincapable of efficiently inducing mammalian RNAi. Certain highlyfunctional siRNAs designed for knockout of a particular gene mayrender unrelated endogenous genes nonfunctional. These majorbottlenecks should be properly eliminated when RNAi technologiesare employed for any experiment in mammalian functional genomics.This paper thus presents essential notes and findings regardingthe proper choice of siRNA-sequence selection algorithms andweb-based online software systems

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