Decreased Expression Of apM1 in Omental and Subcutaneous Adipose Tissue of HumansWith Type 2 Diabetes | Zendy

Michael A. Statnick | Zendy; Lisa S. Beavers | Zendy; Laura J. Conner | Zendy; Helena Corominola | Zendy; D. Brooke Johnson | Zendy; Craig D. Hammond | Zendy; Ronit Rafaeloff-Phail | Zendy; Thomas Seng | Zendy; Todd M. Suter | Zendy; James P. Sluka | Zendy; Éric Ravussin | Zendy; Robert A. Gadski | Zendy; F. José | Zendy

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Decreased Expression Of apM1 in Omental and Subcutaneous Adipose Tissue of HumansWith Type 2 Diabetes

Author(s) -

Michael A. Statnick,

Lisa S. Beavers,

Laura J. Conner,

Helena Corominola,

D. Brooke Johnson,

Craig D. Hammond,

Ronit Rafaeloff-Phail,

Thomas Seng,

Todd M. Suter,

James P. Sluka,

Éric Ravussin,

Robert A. Gadski,

F. José

Publication year - 2000

Publication title -

journal of diabetes research

Language(s) - English

Resource type - Journals

eISSN - 2314-6753

pISSN - 2314-6745

DOI - 10.1155/edr.2000.81

Subject(s) - adipose tissue , subcutaneous adipose tissue , type 2 diabetes , subcutaneous fat , endocrinology , medicine , diabetes mellitus , biology , microbiology and biotechnology , psychology , cancer research

We have screened a subtracted cDNA library in order to identify differentially expressed genes in omental adipose tissue of human patients with Type 2 diabetes. One clone (#1738) showed a marked reduction in omental adipose tissue from patients with Type 2 diabetes. Sequencing and BLAST analysis revealed clone #1738 was the adipocyte-specific secreted protein gene apM1 (synonyms ACRP30, AdipoQ, GBP28). Consistent with the murine orthologue, apM1 mRNA was expressed in cultured human adipocytes and not in preadipocytes. Using RT-PCR we confirmed that apM1 mRNA levels were significantly reduced in omental adipose tissue of obese patients with Type 2 diabetes compared with lean and obese normoglycemic subjects. Although less pronounced, apM1 mRNA levels were reduced in subcutaneous adipose tissue of Type 2 diabetic patients. Whereas the biological function of apM1 is presently unknown, the tissue specific expression, structural similarities to TNFalpha, and the dysregulated expression observed in obese Type 2 diabetic patients suggest that this factor may play a role in the pathogenesis of insulin resistance and Type 2 diabetes.

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